National Institute on Drug Abuse - Intramural Research Program
Featured Paper of the Month – May 2023
Published in PNAS with contributions from Carl Lupica, Alexander Hoffman, and Michael Baumann of the NIDA IRP.
Here we demonstrate that various DAT inhibitors can be distinguished based on their in vitro binding kinetics, whereby the dissociation rate (i.e., Koff) of a drug from DAT in vitro predicts the duration of its stimulant effects in vivo.
Reviews To Read – April 2023. Published in Medical Research Reviews with contributions from Alessandro Bonifazi and Lily Saab of the NIDA IRP Medicinal Chemistry Section. This review, written in collaboration between authors from the Medicinal Chemistry Section (NIDA-IRP) and the Medicinal Chemistry and Pharmacology Units at the University of Camerino (Italy), is focused on the development of… [Read More]
Insights into distinct signaling profiles of the µOR activated by diverse agonists. Nat Chem Biology.
The TRUPATH BRET2 assay is a single and comprehensive platform of 14 G protein biosensors that allows for unambiguous measurement of heterotrimeric G protein dissociation following agonist-induced receptor stimulation. Because individual G protein subunits have distinct functional characteristics, directly monitoring their activation and signaling profiles provides new opportunities for revealing previously uncharacterized actions of GPCR ligands. [Read More]
Featured Paper of the Month – April 2023
Published in Science Advances by Oscar Solís and Michael Michaelides et al. from the NIDA IRP Biobehavioral Imaging and Molecular Neuropsychopharmacology Unit.
In our study, we investigated the potential impact of the spike protein of the SARS-CoV-2 virus on estrogen receptors. To do so, we used computational and biochemical methods to study the binding of the spike protein to human estrogen receptors…
Hot Off the Press – March 28, 2023 Published in Nature Neuroscience by Kauê Machado Costa and Geoffrey Schoenbaum of the NIDA IRP Behavioral Neurophysiology Neuroscience Section. Summary We use mental models of the world – also called cognitive maps – to guide behavior. Several mental illnesses, including schizophrenia and substance use disorder, involve disruptions… [Read More]
Hot Off the Press – March 16, 2023 Summary The brain µ-opioid receptor (MOR) is critical for the analgesic, rewarding, and addictive effects of opioid drugs. However, in rat models of opioid-related behaviors, the circuit mechanisms of MOR-expressing cells are less known because of a lack of genetic tools to selectively manipulate them. We introduce… [Read More]
Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging. Cell.
A group of researchers from Stanford University employed a multiplexed mass cytometry to profile the epigenetic landscape and measure a broad array of global chromatin modifications in human cells at the single-cell level. The EpiTOF technology provides new opportunities for identifying cell-specific epigenetic changes associated with altered physiological and pathological states. [Read More]
Featured Paper of the Month – March 2023
Published in PNAS by Ying Duan, Pei-Jung Tsai and Yihong Yang, et al. from the NIDA IRP Magnetic Resonance Imaging and Spectroscopy Section.
Compulsive drug use, as the defining feature of substance use disorder, is attributed to disadvantageous decision-making and has been associated with dysfunction of frontal-midbrain systems. The habenula is a relay node between forebrain and midbrain regions and processes negative feedback in response to aversive events. To understand the contributions of frontal-habenula-midbrain circuits in the development of drug dependence, we employed a rat model of methamphetamine self-administration in the presence of concomitant footshock, which has been proposed to model compulsive drug-taking in humans…
Dr. Morales was chosen to receive a 2023 Winter Conference on Brain Research (WCBR) Pioneer Award for her distinguished career as chief of the Integrative Neuroscience Research Branch and Neuronal Networks Section at the NIDA IRP. Congratulations Dr. Morales, from all of us at the NIDA IRP! From the WCBR Website: “Pioneer Awards were introduced… [Read More]
Polony gels enable amplifiable DNA stamping and spatial transcriptomics of chronic pain. Cell.
Fu*, Sun*, Dong* et. al. generated and validated a novel polony-based ‘stamp gel’ that uses common lab equipment and enzymatic replication to produce copies of the 1 µm resolution barcoded array slide that is used for spatial transcriptomic capture. Taken together, polony gel stamping is poised to increase accessibility of high-resolution spatial transcriptomics. [Read More]
