The TAC provides training and access to the following lab equipment: 96-well plate shakers, centrifuges, homogenizers, microplate readers, fume hoods, biological safety cabinet, pipettes, and microplate readers. The TAC also has equipment marked with (*) for which we offer full-service analysis but are only operated by TAC staff.
Access to equipment: The TAC bench laboratory is located in the NIDA IRP, BRC, Rm. 01B216. To reserve equipment or lab bench space please sign up on the TAC Calendar. As a BSL-2 lab, users are required to have completed all appropriate safety training to gain access to the lab.
Protein Simple ELLA system is a microplate reader that conducts immunoassays using a microfluidic Simple Plex cartridge. The use of these cartridges simplifies the ELISA protocol required for analysis. Results can be obtained in approximately 90 minutes. Protein Simple offers several assays on the Simple Plex cartridge. A list of current analytes that can be measured can be found here.
Protein Simple ELLA system
BMG LabTech CLARIOstar Plus is a high-performance multi-mode plate reader. It is compatible with most commercially available EIA/ELISA kits. Please reach out to TAC staff if you would like assistance in selecting the appropriate EIA/ELISA kit for your needs.
CLARIOstar Plus
*Agilent GC/MS system consists of an 8890 gas chromatograph coupled to a 5977B Inert Plus MSD mass analyzer with Extractor El source. This instrument is used to analyze and measure small biomolecules (eg. drugs, alcohol, metabolites, etc.) in a wide range of biological matrices (e.g. plasma, urine, etc.). The system comes with two inlets: one attached to a7693A Autoinjector for liquid introduction and the other to a 7697A Headspace Sampler for measuring BAL from whole blood or plasma.
Agilent GC/MS system
*Thermo Fisher Scientific Orbitrap Exploris 120 mass spectrometer is a high-resolution FT bench top system. The instrument is well-suited for exact mass measurements with a mass resolution up to 120,000 (FWHM) at m/z 200 and a mass accuracy down to < 1 ppm RMS with internal calibration. MS/MS analysis allows for structural identification of compounds. The mass spectrometer is coupled to a Vanquish Horizon / Flex liquid chromatograph for the analysis of drug molecules and for conducting lipidomic and metabolomic studies.
Thermo Fisher Scientific Orbitrap Exploris 120 mass spectrometer
