Email: nidatransgenicprojects@mail.nih.gov
Location:
Biomedical Research Center
251 Bayview Blvd
Suite 200
Baltimore, MD 21224
Related Pages
About The Transgenic Rat Project
Welcome to NIDA’s Trangenic Rat Project! The NIDA intramural research program (IRP) with support from IRPs at NIMH, NIAAA, and NINDS have been working over the past few years to generate and characterize the transgenic rats listed below. The goal of the Transgenic Rat project is to create and characterize a panel of rats for use in models of neurological diseases such as addiction and neurodegeneration. As part of the production and sharing of novel transgenic rats, this website will serve as a data repository for users of the transgenic rats.
All users of NIDA OTTC-generated transgenic rats will agree to provide annual feedback (positive and negative) to be placed on the website for existing and potential users to view. The overall goal is to improve the reproducibility of research using transgenic models of disease.
All strains listed below are available at the Rat Resource and Research Center (RRRC). If you are interested in obtaining the rats, please request the strain(s) by following the appropriate link(s) to the corresponding RRRC webpages.
Transgenic Rat Strains
LE-Tg(cFos-eGFP)2Ottc
Request: RRRC#766 
Promoter: cFos
Transgene: eGFP
Notes: Reporter rat expressing eGFP in cFos expressing cells
LE-Tg(cFos-LacZ)1Ottc
Request: RRRC#759
Promoter: cFos
Transgene: LacZ
Notes: Reporter rat expressing beta-galactosidase in cFos expressing cells
LE-Tg(DAT-iCre)6Ottc
Request: RRRC#758
Promoter: DAT (dopamine transporter)
Transgene: iCre
Notes:BAC transgenic using rat DAT promoter to express Cre recombinase in dopaminergic neurons
LE-Tg(DIO-iRFP)3Ottc
Request: RRRC#747
Promoter: EF1alpha
Transgene: iRFP
Notes: Cre recombinase reporter rat on Long Evans (LE) background expressing iRFP
SD-Tg(DIO-iRFP)9Ottc
Request: RRRC#748
Promoter: EF1alpha
Transgene: iRFP
Notes: Cre recombinase reporter rat on Sprague Dawley (SD) background expressing iRFP
LE-Tg(DIO-mCherry)2Ottc
Request: RRRC#687
Promoter: EF1alpha
Transgene: mCherry
Notes: Cre recombinase reporter rat expressing mCherry
LE-Tg(EF1a-TetR)1Ottc
Request: RRRC#761
Promoter: EF1alpha
Transgene: TetR
Notes: Expresses TetR using “ubiquitous” promoter; to be used in combination with TetO containing rats or viral vectors
LE-Tg(Gad1-iCre)3Ottc
Request: RRRC#751
Promoter: GAD1 (glutamate decarboxylase 1)
Transgene: iCre
Notes: BAC transgenic using rat GAD1 promoter to express Cre recombinase in GABAergic neurons
LE-Tg(Drd1a-iCre)3Ottc
Request: RRRC#767
Promoter: Drd1a (Dopamine receptor D1a)
Transgene: iCre
Notes: BAC transgenic using rat dopamine receptor 1 promoter to express Cre recombinase in D1R neurons
LE-Tg(Drd2-iCre)1Ottc
Request: RRRC#768
Promoter: Drd2 (Dopamine receptor D2)
Transgene: iCre
Notes: BAC transgenic using rat dopamine receptor 2 promoter to express Cre recombinase in D2R neurons
LE-Tg(Pvalb-iCre)2Ottc
Request: RRRC#773
Promoter: PVALb (parvalbumin)
Transgene: iCre
Notes: BAC transgenic using rat parvalbumin promoter to express Cre recombinase in parvalbumin expressing neurons
LE-Tg(OTTC1005) CX3CR1-Cre-ERT2
Request: RRRC#858
Promoter: CX3CR1 (chemokine receptor CX3CR1)
Transgene: Cre-ERT2
Notes: BAC transgenic using rat CX3CR1 promoter to express Cre recombinase in microglia
LE-Rosa26Tm1(LSL-Cas9)Ottc
Request: RRRC #833
Promoter: CAG (synthetic hybrid construct consisting of the cytomegalovirus (CMV) enhancer fused to the chicken beta-actin promoter)
Transgene: FLAG-tagged SpCas9 nuclease flanked with nuclear localization signals
Notes: Ubiquitous but Cre-dependent expression of FLAG-tagged SpCas9 nuclease flanked with nuclear localization signals on a Long Evans (LE) background
LE-Rosa26Tm1(LSL-Cas9D10A)Ottc
Request: RRRC # 834
Promoter: CAG (synthetic hybrid construct consisting of the cytomegalovirus (CMV) enhancer fused to the chicken beta-actin promoter)
Transgene: FLAG-tagged SpCas9 (D10A) nickase flanked with nuclear localization signals
Notes: Ubiquitous but Cre-dependent expression of FLAG-tagged SpCas9 (D10A) nickase flanked with nuclear localization signals on a Long Evans (LE) background
SD-Tg(Oprm1-iCre)1Ottc
Request: RRRC # 975
Promoter: Oprm1
Transgene: iCre
Notes: Targeted knocking adding a T2A-iCre to the Oprm1 (mu opioid receptor) in SD rat
