Last Updated on December 16, 2022
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Figure 1: A schematic of the CX3CR1-Cre-ERT2 transgenic construct. A bacterial artificial chromosome (BAC) containing the rat CX3CR1 gene (CH230-274J7) was obtained from the Children’s Hospital Oakland Research Institute (CHORI), and recombineered to replace the start codon of CX3CR1 with a cassette containing Cre-ERT2 (tamoxifen-inducible Cre recombinase) and the polyadenylation signal from the gene for bovine growth hormone. This modified BAC (pOTTC1005) was injected into the pronuclei of fertilized Long Evans rat embryos by Cyagen (China), and ultimately resulted in (two) independent, phenotypically positive lines. The line (LE-Tg(CX3CR1-Cre-ERT2)3Ottc) has between 7-9 copies of the transgene per haploid genome as determined by droplet digital PCR.
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Blog/Comments/ReviewsLast Updated on December 16, 2022
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