AAV-delivered muscone-induced transgene system for treating chronic diseases in mice via inhalation
Published in Nature Communications.
Authors
Xin Wu, Yuanhuan Yu, Meiyan Wang, Di Dai, Jianli Yin, Wenjing Liu, Deqiang Kong, Shasha Tang, Meiyao Meng, Tian Gao, Yuanjin Zhang, Yang Zhou, Ningzi Guan, Shangang Zhao, Haifeng Ye
Paper presented by Dr. Suzanne Underhill and selected by the NIDA TDI Paper of the Month Committee
Publication Brief Description
AAV-mediated gene expression is typically constitutive and constrained by the promoter and other regulatory elements. Ligand inducible transcription systems offer more dynamic expression but the typical antibiotic-sensitive elements can lead to complications with antibiotic resistance as well as cytotoxicity.
To address these concerns, Wu et al developed an odorant-induced expression vector, the muscone-induced transgene system (MUSE). Using the muscone responsive odorant receptor (MOR215-1), they were able to demonstrate muscone induced gene expression that was time- and dose-dependent . Using MUSE they achieved therapeutic effects in murine models of nonalcoholic fatty liver disease (NAFLD) and OVA-induced allergic asthma, targeting the liver and lungs, respectively.
MUSE represents an emerging tool for ligand-inducible transgene expression that could be implemented in substance use disorder and addiction models.
AAV-delivered muscone-induced transgene system for treating chronic diseases in mice via inhalation Journal Article
In: Nat Commun, vol. 15, no. 1, pp. 1122, 2024, ISSN: 2041-1723.
