Last Updated on November 12, 2024
Background | Status & Availability | Transgene Info | Phenotypic Characterization | Breeding | Genotyping | References | Blog/Comments/Reviews | Related rats | Acknowledgements
Background
Status and Availability
This strain has not yet been published.
As of January 3, 2020, this strain is available as line 858 at the RRRC.
This rat is registered at the Rat Genome Database (RGD) as RGD ID# 13441557.
Transgene Information
Figure 1: A schematic of the CX3CR1-Cre-ERT2 transgenic construct. A bacterial artificial chromosome (BAC) containing the rat CX3CR1 gene (CH230-274J7) was obtained from the Children’s Hospital Oakland Research Institute (CHORI), and recombineered to replace the start codon of CX3CR1 with a cassette containing Cre-ERT2 (tamoxifen-inducible Cre recombinase) and the polyadenylation signal from the gene for bovine growth hormone. This modified BAC (pOTTC1005) was injected into the pronuclei of fertilized Long Evans rat embryos by Cyagen (China), and ultimately resulted in (two) independent, phenotypically positive lines. The line (LE-Tg(CX3CR1-Cre-ERT2)3Ottc) has between 7-9 copies of the transgene per haploid genome as determined by droplet digital PCR.
Phenotypic Characterization
Breeding Strategy
Breeding Information, click here for PDF
Genotyping Assays
Assay for the presence of CX3CR1 and CRE-ERT2, click here for PDF
References that cite this rat
No references are available at this time.
Blog/Comments/Reviews
Last Updated on November 12, 2024
No comments or reviews are available at this time.
Other related rats
Acknowledgements
Brandon Harvey, Chris Richie, Lamarque Coke and Janette Lebron
