• Skip to primary navigation
  • Skip to main content
  • Skip to primary sidebar

NIDA IRP

National Institute on Drug Abuse - Intramural Research Program

  National Institute on Drug Abuse | NIH IRP | Treatment Info | Emergency Contacts
  • Home
  • News
    • Featured Paper of the Month
    • Reviews to Read
    • Hot off the Press
    • IRP News
    • Awards
    • Technology Development Initiative Paper of the Month
    • Seminar Series
    • Addiction Grand Rounds
  • About
    • About NIDA IRP
    • Contact Us
    • Directions and Map
    • Careers at NIDA IRP
    • Emergency Contacts
    • Employee Assistance Resources
  • Organization
    • Faculty
    • Office of the Scientific Director
    • Office of the Clinical Director
    • Office of Education and Career Development
    • Administrative Management Branch
    • Molecular Targets and Medications Discovery Branch
    • Cellular and Neurocomputational Systems Branch
    • Molecular Neuropsychiatry Research Branch
    • Neuroimaging Research Branch
    • Behavioral Neuroscience Research Branch
    • Integrative Neuroscience Research Branch
    • Translational Addiction Medicine Branch
    • Core Facilities
    • Community Outreach Group
  • Training Programs
    • Office of Education and Career Development
    • OECD Awards
    • Summer Internship Program
    • Postbaccalaureate Program
    • Graduate Partnership Program
    • Postdoctoral Program
    • NIDA Speakers Bureau
    • Clinical Electives Program
    • Clinical Mentoring Program
  • Study Volunteers
  • Transgenic Rat Home
  • OTTC Information
  • Publications
  • Technology Development Initiative
  • Links

SD-Tg(Oprm1-iCre)1Ottc

Last Updated on November 12, 2024

Background | Status & Availability | Transgene Info | Phenotypic Characterization | Breeding | Genotyping | References | Blog/Comments/Reviews | Related rats | Acknowledgements

Background

The µ-opioid receptor (MOR), also known as OPRM1, is highly expressed in several brain regions and the most targeted opioid receptor for pain management. MOR has several downstream targets including GABA, which is the primary inhibitory neurotransmitter in the mammalian brain. To facilitate the genetic manipulation of MOR expressing cells, we have generated and characterized a transgenic Sprague Dawley rat expressing iCre recombinase under the OPRM1 promoter (OPRM1::iCre, line 1). The tissue-specific expression of iCre can be used to specifically express Cre-dependent in OPRM1(+) neurons.

Status and Availability

This strain is anticipated to be made available Spring 2023 as line 975 at the RRRC. You are now exiting the NIDA IRP Website
The rat is registered at the Rat Genome Database (RGD) as RGD ID#155641245 .

Transgene Information

Figure 1. CRISPR-mediated knock-in of T2A-iCre at the end of rat Oprm1 gene

Figure 1. CRISPR-mediated knock-in of T2A-iCre downstream of the rat Oprm1 coding sequence. Schematic of the target gene (rat Oprm1) with annotation for the location and sequence of the SpCas9 sgRNA that cleaves within the stop codon. The donor template encoding homologous arms and the T2A-iCre transgene are also shown.

 

Phenotypic Characterization

Figure 2. iCre mRNA and Oprm1 mRNA in striatum and dorsal hippocampus (1 of 2)

Figure 2. iCre mRNA and Oprm1 mRNA in striatum and dorsal hippocampus (2 of 2)

 

Figure 2. iCre mRNA and Oprm1 mRNA in nucleus accumbens, dorsal striatum, and dorsal hippocampus. (A) Oprm1+ cells per mm2 for Oprm1 mRNA (wildtype and Oprm1-Cre, n=5/genotype). (B) Oprm1+/Cre+ double labeled cells per mm2 (Oprm1-Cre rats only). (C) Percent Cre+/Oprm1+ (Oprm1-Cre rats only). (D) Representative confocal photomicrographs of Oprm1-Cre rat brains showing colocalization (yellow) between Oprm1+ neurons (red) and Cre+ neurons (green) in dHipp, DS, and NAc in comparison to wildtype rats which only showed Oprm1 expression (red). Objective lens magnification: D: A,C 10X and D: B,D 40X. Scale bars: D: A,C = 300 µm; D: B,D = 25 µm. Abbreviations: anterior commissure, Aca; hippocampal subfields, CA1, CA2, CA3; corpus callosum, cc; dentate gyrus, DG; dorsal striatum, dSTR; left ventricle, lv. For more information see, PMID: 36717230.

Additional phenotypic characterization is recommended for brain region and experimental paradigm of interest.

Breeding Strategy

Breeding Information, click here for PDF

Genotyping Assays

Click here for PDF

References that cite this rat

No references at this time.

Blog/Comments/Reviews

Last Updated on November 12, 2024

No comments or reviews are available at this time.

Other related rats

No related rats at this time.

Acknowledgements

Yavin Shaham, Brandon Harvey, Jennifer Bossert, Chris Richie, Francois Vautier

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Primary Sidebar

Transgenic Rats Project

  • Transgenic Rat Home
  • OTTC Information
  • Publications
  • Technology Development Initiative
  • Links

Organization

  • Organization
  • Faculty
  • Office of the Scientific Director
  • Office of the Clinical Director
  • Administrative Management Branch
  • Molecular Targets and Medications Discovery Branch
  • Cellular and Neurocomputational Systems Branch
  • Molecular Neuropsychiatry Research Branch
  • Neuroimaging Research Branch
  • Behavioral Neuroscience Research Branch
  • Integrative Neuroscience Research Branch
  • Translational Addiction Medicine Branch
  • Core Facilities
  • Careers at NIDA IRP
  • Technology Development Initiative
  • Community Outreach Group
Home / Organization / Office of the Scientific Director / Transgenic Rat Project / SD-Tg(Oprm1-iCre)1Ottc
  • National Institute on Drug Abuse
  • NIH Intramural Research Program
  • National Institutes of Health
  • Health and Human Services
  • USA.GOV
  • Emergency Contacts
  • Employee Assistance
  • Treatment Information
  • Contact Us
  • Careers at NIDA IRP
  • Accessibility
  • Privacy
  • HHS Vulnerability Disclosure
  • Freedom of Information Act
  • Document Viewing Tools
  • Offsite Links
  • National Institute on Drug Abuse
  • NIH Intramural Research Program
  • National Institutes of Health
  • Health and Human Services
  • USA.GOV
  • Emergency Contacts
  • Employee Assistance
  • Treatment Information
  • Contact Us
  • Careers at NIDA IRP
  • Accessibility
  • Privacy
  • HHS Vulnerability Disclosure
  • Freedom of Information Act
  • Document Viewing Tools
  • Offsite Links

  • Home
  • News
    ▼
    • Featured Paper of the Month
    • Reviews to Read
    • Hot off the Press
    • IRP News
    • Awards
    • Technology Development Initiative Paper of the Month
    • Seminar Series
    • Addiction Grand Rounds
  • About
    ▼
    • About NIDA IRP
    • Contact Us
    • Directions and Map
    • Careers at NIDA IRP
    • Emergency Contacts
    • Employee Assistance Resources
  • Organization
    ▼
    • Faculty
    • Office of the Scientific Director
    • Office of the Clinical Director
    • Office of Education and Career Development
    • Administrative Management Branch
    • Molecular Targets and Medications Discovery Branch
    • Cellular and Neurocomputational Systems Branch
    • Molecular Neuropsychiatry Research Branch
    • Neuroimaging Research Branch
    • Behavioral Neuroscience Research Branch
    • Integrative Neuroscience Research Branch
    • Translational Addiction Medicine Branch
    • Core Facilities
    • Community Outreach Group
  • Training Programs
    ▼
    • Office of Education and Career Development
    • OECD Awards
    • Summer Internship Program
    • Postbaccalaureate Program
    • Graduate Partnership Program
    • Postdoctoral Program
    • NIDA Speakers Bureau
    • Clinical Electives Program
    • Clinical Mentoring Program
  • Study Volunteers