Contact
Biomedical Research Center251 Bayview Boulevard
Baltimore, MD 21224
Email: akira.sumiyoshi@nih.gov
Education
B.S. - Pharmaceutical Sciences, Nagoya City University
M.S. - Pharmaceutical Sciences, Nagoya City University
Ph.D. - Medical Sciences, Tohoku University
Research Interests
Dr. Sumiyoshi received his B.S. (2005) and M.S. (2007) in Pharmaceutical Sciences from Nagoya City University, Japan. In 2008, he started pursuing a Ph.D. in Medical Sciences at Tohoku University, Japan, where he helped set-up animal MRI laboratory and developed brain imaging techniques in rodents (simultaneous EEG and fMRI, MRI template atlas, and voxel-based morphometry). After receiving his Ph.D. in 2011, he spent five years as a Research Associate in Tohoku University, Japan, and joined NIDA-IRP, NIH, in 2016 to establish whole brain connectome analysis in rodents using multimodal imaging techniques under supervision of Drs. Lu, Yang, and Stein.
Publications
Selected Publications
2017
Sumiyoshi, Akira; Nonaka, Hiroi; Kawashima, Ryuta
Sexual differentiation of the adolescent rat brain: A longitudinal voxel-based morphometry study. Journal Article
In: Neurosci Lett, vol. 642, pp. 168–173, 2017, ISSN: 1872-7972 (Electronic); 0304-3940 (Linking).
@article{Sumiyoshi2017,
title = {Sexual differentiation of the adolescent rat brain: A longitudinal voxel-based morphometry study.},
author = {Akira Sumiyoshi and Hiroi Nonaka and Ryuta Kawashima},
url = {https://www.ncbi.nlm.nih.gov/pubmed/28188846},
doi = {10.1016/j.neulet.2016.12.023},
issn = {1872-7972 (Electronic); 0304-3940 (Linking)},
year = {2017},
date = {2017-02-07},
journal = {Neurosci Lett},
volume = {642},
pages = {168--173},
address = {Institute of Development, Aging and Cancer, Tohoku University, 4-1, Seiryo-cho, Aoba-ku, Sendai 980-8575, Japan. Electronic address: akira.sumiyoshi.a4@tohoku.ac.jp.},
abstract = {The sexual differentiation of the rat brain during the adolescent period has been well documented in post-mortem histological studies. However, to further understand the morphological changes occurring in the entire brain, a noninvasive neuroimaging method allowing an unbiased, comprehensive, and longitudinal investigation of brain morphology should be used. In this study, we investigated the sexual differentiation of the rat brain during the adolescent period using longitudinal voxel-based morphometry (VBM) analysis. Male and female Wistar rats (n=12 of each) were scanned in a 7.0-T MRI scanner at five time points from 6 to 10 weeks of age. The T2-weighted MRI images were segmented using the rat brain tissue priors that have been published by our laboratory. At the global level, the results of the VBM analysis showed greater increases in total gray matter volume in the males during the adolescent period, although we did not find significant differences in total white matter volume. At the voxel level, we found significant increases in the regional gray matter volume of the occipital cortex, amygdala, hippocampal formation, and cerebellum. At the regional level, only the occipital cortex in the females exhibited decreases during the adolescent period. These results were, at least in part, consistent with those of previous longitudinal VBM studies in humans, thus providing translational evidence of the sexual differentiation of the developing brain between rodents and humans.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The sexual differentiation of the rat brain during the adolescent period has been well documented in post-mortem histological studies. However, to further understand the morphological changes occurring in the entire brain, a noninvasive neuroimaging method allowing an unbiased, comprehensive, and longitudinal investigation of brain morphology should be used. In this study, we investigated the sexual differentiation of the rat brain during the adolescent period using longitudinal voxel-based morphometry (VBM) analysis. Male and female Wistar rats (n=12 of each) were scanned in a 7.0-T MRI scanner at five time points from 6 to 10 weeks of age. The T2-weighted MRI images were segmented using the rat brain tissue priors that have been published by our laboratory. At the global level, the results of the VBM analysis showed greater increases in total gray matter volume in the males during the adolescent period, although we did not find significant differences in total white matter volume. At the voxel level, we found significant increases in the regional gray matter volume of the occipital cortex, amygdala, hippocampal formation, and cerebellum. At the regional level, only the occipital cortex in the females exhibited decreases during the adolescent period. These results were, at least in part, consistent with those of previous longitudinal VBM studies in humans, thus providing translational evidence of the sexual differentiation of the developing brain between rodents and humans.
2014
Sumiyoshi, Akira; Taki, Yasuyuki; Nonaka, Hiroi; Takeuchi, Hikaru; Kawashima, Ryuta
Regional gray matter volume increases following 7days of voluntary wheel running exercise: a longitudinal VBM study in rats. Journal Article
In: Neuroimage, vol. 98, pp. 82–90, 2014, ISSN: 1095-9572 (Electronic); 1053-8119 (Linking).
@article{Sumiyoshi2014,
title = {Regional gray matter volume increases following 7days of voluntary wheel running exercise: a longitudinal VBM study in rats.},
author = {Akira Sumiyoshi and Yasuyuki Taki and Hiroi Nonaka and Hikaru Takeuchi and Ryuta Kawashima},
url = {https://www.ncbi.nlm.nih.gov/pubmed/24816532},
doi = {10.1016/j.neuroimage.2014.04.075},
issn = {1095-9572 (Electronic); 1053-8119 (Linking)},
year = {2014},
date = {2014-05-09},
journal = {Neuroimage},
volume = {98},
pages = {82--90},
address = {Department of Functional Brain Imaging, Institute of Development, Aging and Cancer, Tohoku University, Sendai 980-8575, Japan. Electronic address: sumiyoshi@idac.tohoku.ac.jp.},
abstract = {The effects of physical exercise on brain morphology in rodents have been well documented in histological studies. However, to further understand when and where morphological changes occur in the whole brain, a noninvasive neuroimaging method allowing an unbiased, comprehensive, and longitudinal investigation of brain morphology should be used. In this study, we investigated the effects of 7days of voluntary wheel running exercise on regional gray matter volume (rGMV) using longitudinal voxel-based morphometry (VBM) in rats. Eighteen pairs of adult male naive Wistar rats were randomized to the exercise or control condition (one rat for each condition from each pair). Each rat was scanned in a 7.0-T MRI scanner at three time points: before exercise, after 7days of exercise, and after 7days of follow-up. The T2-weighted MRI images were segmented using the rat brain tissue priors that were recently published by our laboratory, and the intra- and inter-subject template creation steps were followed. Longitudinal VBM analysis revealed significant increases in rGMV in the motor, somatosensory, association, and visual cortices in the exercise group. Among these brain regions, rGMV changes in the motor cortex were positively correlated with the total distance that was run during the 7days of exercise. In addition, the effects of 7days of exercise on rGMV persisted after 7days of follow-up. These results support the utility of a longitudinal VBM study in rats and provide new insights into experience-dependent structural brain plasticity in naive adult animals.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The effects of physical exercise on brain morphology in rodents have been well documented in histological studies. However, to further understand when and where morphological changes occur in the whole brain, a noninvasive neuroimaging method allowing an unbiased, comprehensive, and longitudinal investigation of brain morphology should be used. In this study, we investigated the effects of 7days of voluntary wheel running exercise on regional gray matter volume (rGMV) using longitudinal voxel-based morphometry (VBM) in rats. Eighteen pairs of adult male naive Wistar rats were randomized to the exercise or control condition (one rat for each condition from each pair). Each rat was scanned in a 7.0-T MRI scanner at three time points: before exercise, after 7days of exercise, and after 7days of follow-up. The T2-weighted MRI images were segmented using the rat brain tissue priors that were recently published by our laboratory, and the intra- and inter-subject template creation steps were followed. Longitudinal VBM analysis revealed significant increases in rGMV in the motor, somatosensory, association, and visual cortices in the exercise group. Among these brain regions, rGMV changes in the motor cortex were positively correlated with the total distance that was run during the 7days of exercise. In addition, the effects of 7days of exercise on rGMV persisted after 7days of follow-up. These results support the utility of a longitudinal VBM study in rats and provide new insights into experience-dependent structural brain plasticity in naive adult animals.
2012
Sumiyoshi, Akira; Suzuki, Hideaki; Shimokawa, Hiroaki; Kawashima, Ryuta
Neurovascular uncoupling under mild hypoxic hypoxia: an EEG-fMRI study in rats. Journal Article
In: J Cereb Blood Flow Metab, vol. 32, no. 10, pp. 1853–1858, 2012, ISSN: 1559-7016 (Electronic); 0271-678X (Linking).
@article{Sumiyoshi2012,
title = {Neurovascular uncoupling under mild hypoxic hypoxia: an EEG-fMRI study in rats.},
author = {Akira Sumiyoshi and Hideaki Suzuki and Hiroaki Shimokawa and Ryuta Kawashima},
url = {https://www.ncbi.nlm.nih.gov/pubmed/22828997},
doi = {10.1038/jcbfm.2012.111},
issn = {1559-7016 (Electronic); 0271-678X (Linking)},
year = {2012},
date = {2012-07-25},
journal = {J Cereb Blood Flow Metab},
volume = {32},
number = {10},
pages = {1853--1858},
address = {Department of Functional Brain Imaging, Institute of Development, Aging, and Cancer, Tohoku University, Sendai, Japan. sumiyoshi@idac.tohoku.ac.jp},
abstract = {The effects of oxygen availability on neurovascular coupling were investigated using simultaneous electroencephalography (EEG) and functional magnetic resonance imaging (fMRI), in addition to the monitoring of physiological parameters, in 16 alpha-chloralose-anesthetized rats. Mild hypoxic hypoxia (oxygen saturation=83.6+/-12.1%) induced significant reductions in fMRI responses (P<0.05) to electrical stimulation in the forepaw, but EEG responses remained unchanged. In addition, the changes in oxygen saturation were linearly correlated with the changes in the fMRI responses. These data further emphasize the importance of oxygen availability, which may regulate neurovascular coupling via the oxygen-dependent enzymatic synthesis of messenger molecules.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The effects of oxygen availability on neurovascular coupling were investigated using simultaneous electroencephalography (EEG) and functional magnetic resonance imaging (fMRI), in addition to the monitoring of physiological parameters, in 16 alpha-chloralose-anesthetized rats. Mild hypoxic hypoxia (oxygen saturation=83.6+/-12.1%) induced significant reductions in fMRI responses (P<0.05) to electrical stimulation in the forepaw, but EEG responses remained unchanged. In addition, the changes in oxygen saturation were linearly correlated with the changes in the fMRI responses. These data further emphasize the importance of oxygen availability, which may regulate neurovascular coupling via the oxygen-dependent enzymatic synthesis of messenger molecules.
Sumiyoshi, Akira; Suzuki, Hideaki; Ogawa, Takeshi; Riera, Jorge J; Shimokawa, Hiroaki; Kawashima, Ryuta
Coupling between gamma oscillation and fMRI signal in the rat somatosensory cortex: its dependence on systemic physiological parameters. Journal Article
In: Neuroimage, vol. 60, no. 1, pp. 738–746, 2012, ISSN: 1095-9572 (Electronic); 1053-8119 (Linking).
@article{Sumiyoshi2012b,
title = {Coupling between gamma oscillation and fMRI signal in the rat somatosensory cortex: its dependence on systemic physiological parameters.},
author = {Akira Sumiyoshi and Hideaki Suzuki and Takeshi Ogawa and Jorge J Riera and Hiroaki Shimokawa and Ryuta Kawashima},
url = {https://www.ncbi.nlm.nih.gov/pubmed/22245345},
doi = {10.1016/j.neuroimage.2011.12.082},
issn = {1095-9572 (Electronic); 1053-8119 (Linking)},
year = {2012},
date = {2012-01-08},
journal = {Neuroimage},
volume = {60},
number = {1},
pages = {738--746},
address = {Department of Functional Brain Imaging, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan. sumiyoshi@idac.tohoku.ac.jp},
abstract = {The simultaneous recordings of neuronal and hemodynamic signals have revealed a significant involvement of high frequency bands (e.g., gamma range, 25-70 Hz) in neurovascular coupling. However, the dependence on a physiological parameter is unknown. In this study, we performed simultaneous electroencephalography (EEG) and functional magnetic resonance imaging (fMRI) recordings in 12 Wistar rats using a conventional forepaw stimulation paradigm and concurrently monitored the systemic physiological parameters of the partial pressure of arterial oxygen, partial pressure of arterial carbon dioxide, pH, mean arterial blood pressure, and heart rate through the rat femoral artery. The high frequency bands in the artifact-free EEG signals, especially those in the gamma range, demonstrated a maximum correlation with fMRI signals in the rat somatosensory cortex. A multiple linear regression analysis demonstrated that the correlation coefficient between the gamma power and fMRI signal depended on the actual values of the physiological parameters (R(2)=0.20, p<0.05), whereas the gamma power and fMRI signal by itself were independent. Among the parameters, the heart rate had a statistically significant slope (95% CI: 0.00027-0.0016, p<0.01) in a multiple linear regression model. These results indicate that neurovascular coupling is mainly driven by gamma oscillations, as expected, but coupling or potential decoupling is strongly influenced by systemic physiological parameters, which dynamically reflect the baseline vital status of the subject.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The simultaneous recordings of neuronal and hemodynamic signals have revealed a significant involvement of high frequency bands (e.g., gamma range, 25-70 Hz) in neurovascular coupling. However, the dependence on a physiological parameter is unknown. In this study, we performed simultaneous electroencephalography (EEG) and functional magnetic resonance imaging (fMRI) recordings in 12 Wistar rats using a conventional forepaw stimulation paradigm and concurrently monitored the systemic physiological parameters of the partial pressure of arterial oxygen, partial pressure of arterial carbon dioxide, pH, mean arterial blood pressure, and heart rate through the rat femoral artery. The high frequency bands in the artifact-free EEG signals, especially those in the gamma range, demonstrated a maximum correlation with fMRI signals in the rat somatosensory cortex. A multiple linear regression analysis demonstrated that the correlation coefficient between the gamma power and fMRI signal depended on the actual values of the physiological parameters (R(2)=0.20, p<0.05), whereas the gamma power and fMRI signal by itself were independent. Among the parameters, the heart rate had a statistically significant slope (95% CI: 0.00027-0.0016, p<0.01) in a multiple linear regression model. These results indicate that neurovascular coupling is mainly driven by gamma oscillations, as expected, but coupling or potential decoupling is strongly influenced by systemic physiological parameters, which dynamically reflect the baseline vital status of the subject.
2011
Valdes-Hernandez, Pedro Antonio; Sumiyoshi, Akira; Nonaka, Hiroi; Haga, Risa; Aubert-Vasquez, Eduardo; Ogawa, Takeshi; Iturria-Medina, Yasser; Riera, Jorge J; Kawashima, Ryuta
An in vivo MRI Template Set for Morphometry, Tissue Segmentation, and fMRI Localization in Rats. Journal Article
In: Front Neuroinform, vol. 5, pp. 26, 2011, ISSN: 1662-5196 (Electronic); 1662-5196 (Linking).
@article{Valdes-Hernandez2011,
title = {An in vivo MRI Template Set for Morphometry, Tissue Segmentation, and fMRI Localization in Rats.},
author = {Pedro Antonio Valdes-Hernandez and Akira Sumiyoshi and Hiroi Nonaka and Risa Haga and Eduardo Aubert-Vasquez and Takeshi Ogawa and Yasser Iturria-Medina and Jorge J Riera and Ryuta Kawashima},
url = {https://www.ncbi.nlm.nih.gov/pubmed/22275894},
doi = {10.3389/fninf.2011.00026},
issn = {1662-5196 (Electronic); 1662-5196 (Linking)},
year = {2011},
date = {2011-11-24},
journal = {Front Neuroinform},
volume = {5},
pages = {26},
address = {Department of Neuroimaging, Cuban Neuroscience Center Havana, Cuba.},
abstract = {Over the last decade, several papers have focused on the construction of highly detailed mouse high field magnetic resonance image (MRI) templates via non-linear registration to unbiased reference spaces, allowing for a variety of neuroimaging applications such as robust morphometric analyses. However, work in rats has only provided medium field MRI averages based on linear registration to biased spaces with the sole purpose of approximate functional MRI (fMRI) localization. This precludes any morphometric analysis in spite of the need of exploring in detail the neuroanatomical substrates of diseases in a recent advent of rat models. In this paper we present a new in vivo rat T2 MRI template set, comprising average images of both intensity and shape, obtained via non-linear registration. Also, unlike previous rat template sets, we include white and gray matter probabilistic segmentations, expanding its use to those applications demanding prior-based tissue segmentation, e.g., statistical parametric mapping (SPM) voxel-based morphometry. We also provide a preliminary digitalization of latest Paxinos and Watson atlas for anatomical and functional interpretations within the cerebral cortex. We confirmed that, like with previous templates, forepaw and hindpaw fMRI activations can be correctly localized in the expected atlas structure. To exemplify the use of our new MRI template set, were reported the volumes of brain tissues and cortical structures and probed their relationships with ontogenetic development. Other in vivo applications in the near future can be tensor-, deformation-, or voxel-based morphometry, morphological connectivity, and diffusion tensor-based anatomical connectivity. Our template set, freely available through the SPM extension website, could be an important tool for future longitudinal and/or functional extensive preclinical studies.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Over the last decade, several papers have focused on the construction of highly detailed mouse high field magnetic resonance image (MRI) templates via non-linear registration to unbiased reference spaces, allowing for a variety of neuroimaging applications such as robust morphometric analyses. However, work in rats has only provided medium field MRI averages based on linear registration to biased spaces with the sole purpose of approximate functional MRI (fMRI) localization. This precludes any morphometric analysis in spite of the need of exploring in detail the neuroanatomical substrates of diseases in a recent advent of rat models. In this paper we present a new in vivo rat T2 MRI template set, comprising average images of both intensity and shape, obtained via non-linear registration. Also, unlike previous rat template sets, we include white and gray matter probabilistic segmentations, expanding its use to those applications demanding prior-based tissue segmentation, e.g., statistical parametric mapping (SPM) voxel-based morphometry. We also provide a preliminary digitalization of latest Paxinos and Watson atlas for anatomical and functional interpretations within the cerebral cortex. We confirmed that, like with previous templates, forepaw and hindpaw fMRI activations can be correctly localized in the expected atlas structure. To exemplify the use of our new MRI template set, were reported the volumes of brain tissues and cortical structures and probed their relationships with ontogenetic development. Other in vivo applications in the near future can be tensor-, deformation-, or voxel-based morphometry, morphological connectivity, and diffusion tensor-based anatomical connectivity. Our template set, freely available through the SPM extension website, could be an important tool for future longitudinal and/or functional extensive preclinical studies.
2010
Sumiyoshi, Akira; Riera, Jorge J; Ogawa, Takeshi; Kawashima, Ryuta
A mini-cap for simultaneous EEG and fMRI recording in rodents. Journal Article
In: Neuroimage, vol. 54, no. 3, pp. 1951–1965, 2010, ISSN: 1095-9572 (Electronic); 1053-8119 (Linking).
@article{Sumiyoshi2010,
title = {A mini-cap for simultaneous EEG and fMRI recording in rodents.},
author = {Akira Sumiyoshi and Jorge J Riera and Takeshi Ogawa and Ryuta Kawashima},
url = {https://www.ncbi.nlm.nih.gov/pubmed/20920590},
doi = {10.1016/j.neuroimage.2010.09.056},
issn = {1095-9572 (Electronic); 1053-8119 (Linking)},
year = {2010},
date = {2010-10-30},
journal = {Neuroimage},
volume = {54},
number = {3},
pages = {1951--1965},
address = {Institute of Development, Aging and Cancer, Tohoku University, Aoba-ku, Sendai, Japan.},
abstract = {Simultaneous recording of electroencephalography (EEG) and functional magnetic resonance imaging (fMRI) is now widely accepted as a prevailing tool to study brain functions. For over a decade, EEG caps with high-dense arrays of electrodes for EEG-fMRI studies in humans have been commercially available. However, simultaneous EEG and fMRI recording in rodents has been limited to only a few electrodes due mainly to two technical reasons, i.e. a small available scalp area and the proximity of the electrodes to the brain tissue. In this paper, we introduce both a new EEG mini-cap and a protocol to obtain whole scalp EEG recordings simultaneously with 7 T fMRI signals in rodents. We provide methodological protocol to evaluate a number of problems emerging from the particulars of using rodents in simultaneous EEG and fMRI recording. The quality and reproducibility of both EEG and fMRI signals were demonstrated using a conventional forepaw stimulation paradigm in Wistar rats. Based on this quantitative analysis, we conclude that simultaneous EEG-fMRI recordings are achievable in rodents without significant signal loss. In light of the contemporary transgenic models and advanced drug administration protocols in rodents, the proposed methodology could be remarkable as a futurist experimental platform.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Simultaneous recording of electroencephalography (EEG) and functional magnetic resonance imaging (fMRI) is now widely accepted as a prevailing tool to study brain functions. For over a decade, EEG caps with high-dense arrays of electrodes for EEG-fMRI studies in humans have been commercially available. However, simultaneous EEG and fMRI recording in rodents has been limited to only a few electrodes due mainly to two technical reasons, i.e. a small available scalp area and the proximity of the electrodes to the brain tissue. In this paper, we introduce both a new EEG mini-cap and a protocol to obtain whole scalp EEG recordings simultaneously with 7 T fMRI signals in rodents. We provide methodological protocol to evaluate a number of problems emerging from the particulars of using rodents in simultaneous EEG and fMRI recording. The quality and reproducibility of both EEG and fMRI signals were demonstrated using a conventional forepaw stimulation paradigm in Wistar rats. Based on this quantitative analysis, we conclude that simultaneous EEG-fMRI recordings are achievable in rodents without significant signal loss. In light of the contemporary transgenic models and advanced drug administration protocols in rodents, the proposed methodology could be remarkable as a futurist experimental platform.
