Christopher Richie, Ph.D.
A NIDA IRP Core Facility
Core Manager
Contact
Location:
Biomedical Research Center
251 Bayview Blvd
Suite 200
Baltimore, MD 21224
Phone: 443-740-2568
Email: gevvc@nida.nih.gov
About the Core
Mission: The Genetic Engineering and Viral Vector Core (GEVVC) provides support to NIDA-IRP researchers by facilitating studies of brain function under physiological and pathological conditions through developing and producing genetic tools capable of modulating and monitoring the molecules, cells and circuits in the nervous system.
Services:
The NIDA GEVVC provides services in three main categories: 1) the custom design and/or production of viral vectors/nanoparticles, 2) in vitro molecular and cell biology services, 3) consultation and training.
Recombinant Plasmids
- Construction, preparation, sequencing, and curation
- Functional testing by transfection into cultured cells
- Ectopic expression and purification of proteins (bacteria/mammalian cell culture)
Adeno-associated Virus (AAV) Technology
- Viral genome design and construction
- Vector packaging (serotypes AAV1, AAV2, rAAV2-retro, AAV6, AAV8, AAV9, AAV-PHP.eB)
- Functional testing by transduction into rat primary cortical neurons.
Lentivirus (LV) Technology
- Viral genome design and construction
- Vector packaging (VSV-G pseudotype)
- Functional testing by transduction into rat primary cortical neurons.
- Transduction and selection to create stably-transduced cell lines
Genome Editing
- Consultation and design of reagents for targeted mutagenesis in rodent models
- Development of genotyping assays
- Quantification of transgene copy number
- BAC recombineering
Training
- Introduction and instruction for “wet bench” molecular biology techniques
- Proper use and safety training for GEVVC-associated capital equipment
